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Simultaneous determination of ketanserin and ketanserinol in biological fluids using ion pair liquid chromatography and fluorometric detection
Summary
Type:
HPLC
Application #:
104040
Detection:
fluorescence at pH 5.2, excitation 310nm, emission 370nm, slit width 10µm
Conditions:
eluent: 2% acetic acid - 0.17M ammonium acetate - acetonitrile (50:10:40)
flow rate 1.2ml/min
pressure 250atm
temperature: 32°C
Authors:
Kurowski, M.
Source:
J. Chromatogr. 341 (1985) 208–212
Details
Legend: Fig. 2. Chromatograms of 1. ketanserinol, 2. ketanserin and 3. internal standard, A) standard mixture of 3ng 1, 3ng 2 and 20ng 3, B) human blank plasma sample spiked with 5ng 1, 5ng 2 and 5ng 3, C)
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